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    In rats, anabolic steroids also act in the peripheral metabolism of thyroid hormones and seem to exert an important proliferative effect on thyroid cellsvia activation of the nuclear factor κB pathway (Lorich et al., 2008). In one study we have revealed a major link between the production of estrogen by steroid receptors and the release of steroids (Kim et al., 2008). In addition, in both estrogen responsive estrogen receptor‐positive (ER+) and -negative breast tumours, steroid response elements in the promoter of the estrogen receptor gene (ERg) can be predicted using human prostate tumor cell lines (Rikschöf et al., 2010; Kallio et al., 2012). This is interesting and a clear demonstration that steroids play an important role in the prostate cancer process.

    In addition to the main hormones, steroids directly affect different aspects of the cell body which are directly related to growth. Steroid hormones such as testosterone, estradiol and progesterone also affect the cell cycle and the differentiation of key cell lineages such as breast and other peripheral tissues. Estrogens are important for the control of ovarian cell proliferation and the development of the mammary glands. The hormone progesterone, which is naturally produced in the human female, also acts in the development of the uterus and ovaries and regulates the function of these cell groups. In human breast cancer cells treated with estrogens, there is a change in the regulation of the matrix metalloproteinases (MMPs), proteins that degrade proteins in the cell wall. This results in the breakdown of the cell wall and increased cell proliferation (Fernandez‐Gonzalez, 2007; Tardif et al., 2010). The MMP‐2, a MMP present in breast carcinoma cells, undergoes apoptosis which contributes to the cell cycle arrest (Bianquen et al., 1999; Al‐Najjar et al., 2006; Sousa et al., 2010). However, in other cancer cell lines, the MMP‐2 does not undergo apoptosis and continues growth (Bianquen et al., 1999; Al‐Najjar et al., 2006).

    The influence of steroids on the functions of cells has profound effects on the overall morphology of the tumour. The cell cycle in these cells becomes more prolonged, and the cellular size is increased with a progressive accumulation of proteins in the cell surface, suggesting that steroids are implicated in the increase in cell surface protein synthesis (Dobrecht and Bijur et al., 2009; Bijur et al., 2012). This also occurs in other breast cancer cells such as PC-3 and

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